About Janus Green B
Janus Green B:-
Janus Green B is one of the important stains that are widely used in histology field. It works as the basic dye in the clinical and laboratory processes. As a leading manufacturer and exporter, we offer the best quality Janus Green B to our customers in medical and pathological research field. This product is also used for staining mitochondria, which is the powerhouse of cells. The staining help in detecting the mitochondria where the color of indicator Janus Green B changes, contingent upon the amount of oxygen available. When the oxygen is present, the indicator is oxidized into blue and comes across as pink in the absence of oxygen. Some attributes of this staining dye is as given below:
- Ensures precise results
- Accurate composition
- Suitable for lab processes
- Available in bulk at industry leading prices
MOQ 50kg
| Specification |
| Chemical Name | 3-(Diethylamino)-7 [{4-dimethylamino)phenyl} azo ] -5-Phenylphenazinium Chloride |
| C.I.No. | 2869-83-2 |
| C.A.S. NO | 11050 |
| Chemical Formula | C30H31ClN6 |
| Molecular Weight | 511.07 gm/mole |
| Dye Content (Spectrophotometry) | >65% |
| Absorption Max (50% Ethanol) | 651 - 678 nm |
| Absorptivity (A1%/1cm., in 50% Ethanol) | > 550 |
| Loss On Drying (110° c) | < 5% |
Reliable Biological StainingJanus Green B is recognized for its efficiency in staining mitochondria and serving as a robust biological indicator dye. Its high purity and laboratory-grade quality ensure sharp, reliable results. The dye is suitable for various routine and diagnostic applications in biology and biochemistry laboratories, supporting precise and reproducible analysis.
Safe Handling and StorageAs a light-sensitive compound, Janus Green B should be stored in a cool, dry place away from light to maintain its stability and effectiveness. Packaged securely in HDPE bottles or sealed containers, it is protected against environmental effects, guaranteeing a shelf life of up to 36 months from the manufacturing date.
Versatile Laboratory ApplicationsWith its solubility in water and ethanol, Janus Green B is easy to prepare for a range of staining techniques. It is widely used in identifying mitochondria and as an indicator in biological research. Its approximate pH level of 6.07.0 (in a 1% solution) is ideal for compatibility with most biological samples.
FAQs of Janus Green B:
Q: How should Janus Green B be properly stored for maximum stability?
A: Janus Green B should be stored in a cool, dry place, protected from direct light. Use the original HDPE bottle or sealed container to help preserve the dyes stability and ensure its 36-month shelf life.
Q: What is the primary use of Janus Green B in laboratory settings?
A: Janus Green B is primarily utilized for biological staining, particularly for visualizing mitochondria in cells. It is also used as an indicator dye for various biological applications.
Q: When should Janus Green B be used in the staining process?
A: Use Janus Green B during the cell staining step, typically after preparing and fixing your specimen. Its action is especially useful for highlighting mitochondria under microscopic examination.
Q: Where can Janus Green B be purchased in India and what are the available pack sizes?
A: Janus Green B is available through authorized dealers, exporters, manufacturers, and suppliers across India. It is supplied in 10 g, 25 g, and 100 g packs, catering to different laboratory needs.
Q: What is the typical process for preparing a Janus Green B staining solution?
A: To prepare a staining solution, dissolve the required quantity of Janus Green B in water or ethanol, according to your protocol. Use a pH within the recommended range of 6.07.0 for best results and handle under subdued light to prevent degradation.
Q: Is Janus Green B safe for all types of laboratory analyses?
A: Janus Green B is intended strictly for laboratory use as an AR/Laboratory Grade dye. It is suitable for standard biological staining, but always consult your laboratorys safety protocols before use.
Q: What are the benefits of using Janus Green B over other dyes?
A: Janus Green B offers high staining specificity for mitochondria, is easy to handle due to its solubility and odorless nature, and has a long shelf life when stored properly. These attributes make it a preferred choice for mitochondrial studies and biological assays.